High-content screening image dataset and quantitative image analysis of Salmonella infected human cells

Automated image analysis for high-content screening and analysis.

The field of high-content screening and analysis consists of a set of methodologies for automated discovery in cell biology and drug development using large amounts of image data. In most cases, imaging is carried out by automated microscopes, often assisted by automated liquid handling and cell culture. Image processing, computer vision, and machine learning are used to automatically process h...

Methods for High-Content, High-Throughput Image-Based Cell Screening

Visual inspection of cells is a fundamental tool for discovery in biological science. Modern robotic microscopes are able to capture thousands of images from massively parallel experiments such as RNA interference (RNAi) or small-molecule screens. Such screens also benefit from lab automation, making large screens, e.g., genome-scale knockdown experiments, more feasible and common. As such, the...

Quantitative assessment of neurite outgrowth in human embryonic stem cell-derived hN2 cells using automated high-content image analysis.

Throughout development neurons undergo a number of morphological changes including neurite outgrowth from the cell body. Exposure to neurotoxic chemicals that interfere with this process may result in permanent deficits in nervous system function. Traditionally, rodent primary neural cultures and immortalized human and non-human clonal cell lines have been used to investigate the molecular mech...

Image-Based High-Content Screening in Drug Discovery

High-Content, Image-Based Screening for Drug Targets in Yeast

BACKGROUND Drug discovery and development are predicated on elucidation of the potential mechanisms of action and cellular targets of candidate chemical compounds. Recent advances in high-content imaging techniques allow simultaneous analysis of a range of cellular events. In this study, we propose a novel strategy to identify drug targets by combining genetic screening and high-content imaging...